Coding
anti-CD19

Part:BBa_K2549005:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-02)


anti-CD19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 444


Design Notes

The heavy-chain variable region (αCD19a) and light-chain variable region (αCD19b) were fused together using a glycine-rich peptide linker (3 repeats of GGGGS, or G4S)[1].

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ Chimeric Antigen Receptor-Modified T Cells in Chronic Lymphoid Leukemia; Chimeric Antigen Receptor-Modified T Cells for Acute Lymphoid Leukemia; Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia. N Engl J Med, 2016 Mar;374(10):998 PMID: 26962747; DOI: 10.1056/NEJMx160005