Part:BBa_K2549002:Design

anti-GFP (LaG2)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Anti-GFP (LaG2) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. LaG2 fuses two copies of LaG16 (Part:BBa_K2549002) using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP^[1].

Source

From IDT (gBlock), codon optimized for human

References

↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170

[1] A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170

[1]