Device

Part:BBa_K2548006

Designed by: Zhuoyi Yang   Group: iGEM18_BNDS_CHINA   (2018-09-30)


Prhl (LR)- RBS - AHL lactonase - Terminator

AHL-lactonase coding device regulated by C4-HSL.

Construction of Quorum Quenching Device I (BBa_K2548000 + BBa_K2548006)

800px-Quorumquenchingdevice1.png

Characterization of Quorum Quenching Device I

Fig.1 Quorum quenching results

We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 110
    Illegal NgoMIV site found at 695
    Illegal AgeI site found at 230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 200


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Parameters
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