Coding

Part:BBa_K2548004:Design

Designed by: Zhuoyi Yang   Group: iGEM18_BNDS_CHINA   (2018-09-30)


AHL-lactonase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 616
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 121


Design Notes

It has been codon optimized by iGEM18_BNDS_CHINA


Source

Pedroza. C. J et.al (2013). Enzymatic hydrolysis of molecules associated with bacterial quorum sensing using an acyl homoserine lactonase from a novel Bacillus thuringiensis strain. Antonie Van Leeuwenhoek, 105(1):253-64. doi: 10.1007/s10482-013-0072-5

References