Regulatory

Part:BBa_K2543000

Designed by: CHUNG-HSUAN HSIAO   Group: iGEM18_Mingdao   (2018-10-08)


Ac5 promoter / pSB1C3

Ac5 is a strong and constitutive promoter from Drosophila actin 5c gene and commonly used in insect expression system.

Ac5 Promoter with Strong Activity

To test the expression vector driven by Ac5 promoter, we cultured a mosquito Aedes albopictus C6/36 cell line and transfected cells with the plasmid of Ac5-GFP-polyA. GFP positive cells and intensity were analyzed 2 days after transfection.

T--Mingdao--qam4.png

T--Mingdao--qam3.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2533


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