Regulatory

Part:BBa_K2540014

Designed by: Anna Guseva   Group: iGEM18_Rice   (2018-10-09)


Orthogonal ribosome binding site

This orthogonal RBS was designed using RBS calculator for an orthogonal 16S rRNA developed by Chubiz & Rao.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 46


References

Chubiz, L. M., & Rao, C. V. (2008). Computational design of orthogonal ribosomes. Nucleic Acids Research, 36(12), 4038–4046.

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