Composite
Part:BBa_K2539300:Design
Designed by: Justin Wu Group: iGEM18_TAS_Taipei (2018-09-03)
AlcR Expression Construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2611
Illegal BamHI site found at 1615
Illegal BamHI site found at 2353 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1319
Illegal AgeI site found at 1447
Illegal AgeI site found at 1978 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
This construct was built to overexpress alcR, a regulatory protein necessary for the activation of PalcA. We acquired all parts from the iGEM distribution kit: a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, strong RBS (BBa_B0034), alcR (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription.