Coding

Part:BBa_K2535002

Designed by: Vignesh Valaboju   Group: iGEM18_Virginia   (2018-10-09)


YdgG

YdgG is a membrane transport protein, not native to the Lsr Operon, which exports AI-2 out of the cell (Herzberg et al 2006). This part can be easily PCR amplified out of E. coli DH5-alpha cells using a colony PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 921
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None