Reporter
Part:BBa_K2525008:Design
Designed by: Kshitij Rai Group: iGEM17_IIT_Delhi (2017-10-27)
PsrpR + RBS + GFP + T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 738
Design Notes
Since the part was Biobrick RFC10 compatible, we did not face any significant issues with the design.
Source
The part (SrpR Repressible promoter) comes from the genomic sequence of Pseudomonas putida