Part:BBa_K2520015

CMV-Mono Display:celiac 1-hGH

This device is a complete system for displaying proteins on cells' membrane. The IgK leader (BBa_K2520024) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (BBa_K2520026) is a trans-membrane domain that anchors all the components located between the IgK leader and the PDGFR itself to the membrane. HA (BBa_K2520030) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of celiac disease (BBa_K2520043) and is component related to our specific project. The CMV promoter (BBa_K1119006) is a constitutive expression promoter in mammalian cells and hGH (BBa_K404108) serves as a terminator.

Celiac

Celiac is a long term autoimmune disorder affecting the internal intestine that is caused due to T cells attacking the Gliadin which is absorbed in the inner wall cells of the small intestine. Gliadin is one of a two proteins that forms gluten. This process leads to severe inflammation, and thus interferes with the absorption of food and greatly increases the chances of contracting colorectal cancer. The prevalence of celiac disease is approximately 0.5% -1% in different parts of the world. The only treatment that eliminates all symptoms (but does not cure the disease) is a strict avoidance of foods containing gluten.

Epitopes

a-Glaiadin 33-mer: a-gliadins in hexaploid wheat are between 25 and 150 copies. The a-gliadin 33-mer is one of the digestion-resistant gluten peptides that is highly reactive to isolated celiac T cells and is the main immunodominant toxic peptide in celiac patients.

References

(1) Gujral, Naiyana, Hugh J. Freeman, and Alan BR Thomson. "Celiac disease: prevalence, diagnosis, pathogenesis and treatment." World journal of gastroenterology: WJG 18.42 (2012): 6036.‏

(2) Ozuna, Carmen V., et al. "Diversification of the celiac disease α‐gliadin complex in wheat: a 33‐mer peptide with six overlapping epitopes, evolved following polyploidization." The Plant Journal 82.5 (2015): 794-805.‏ -- Add more about the biology of this part here

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 614
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 679
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1381