Part:BBa_K2520001
Single Chain Fragment Variable (scFv) of rat anti-murine IgM antibody
Single Chain Fragment Variable (scFv) A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide. This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.
This fusion protein can be secreted or displayed on the cellular membrane.
Results
Our scFv functions as anti-IgM. In order to verify that the anti-IgM construct is functional, we expressed it under two promoters- a mutant EF1a promoter BBa_K2520023 and CMV promoter and we tested the functionality using soluble murine IgM conjugated to a fluorophore.
References
Lee, Timothy K., et al. "Production of engineered IgM-binding single-chain antibodies in Escherichia coli." Journal of Industrial Microbiology & Biotechnology 14.5 (1995): 371-376. Sequence and Features
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- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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