Protein_Domain

Part:BBa_K2520000

Designed by: Dana Kadosh, Noa Eden   Group: iGEM17_TECHNION-ISRAEL   (2017-10-24)


B7-1 Murine Trans Membrane Domain

The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR.

Figure 1: Membrane-anchored single-chain antibodies. A scFv is composed of the immunoglobulin heavy and light chain variable regions linked together with a flexible peptide (normally 15 amino acids in length). For expression of a membrane-anchored scFv, a signal peptide (SP) directs the nascent chain to the lumen of the endoplasmic reticulum whereas the TM anchors the antibody in the plasma membrane.

References

Cheng, Tian‐Lu, and Steve Roffler. "Membrane‐tethered proteins for basic research, imaging, and therapy." Medicinal research reviews 28.6 (2008): 885-928.‏


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None