Coding

Part:BBa_K2519000:Design

Designed by: ching song   Group: iGEM17_UST_Beijing   (2017-10-19)


human ginseng squalene cyclase chimera with built-in human codon bias


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 139
    Illegal NgoMIV site found at 304
    Illegal NgoMIV site found at 1340
    Illegal NgoMIV site found at 1969
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A few amino acid substitutions are made to avoid possible structural space overlap.


Source

total synthesis

References