Coding

Part:BBa_K2515003:Design

Designed by: Slavil Peykov   Group: iGEM17_Bulgaria   (2017-10-27)


Inverted INPUT TetR generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes



This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.

To clone this part by yourself, use the following primer pair:

Tet_Gen_InvF TATCTGCAGCGGCCGCTACTAGTAttgacagctagctcagtcc
Tet_Gen_InvR TATGAATTCGCGGCCGCTTCTAGAGTTAcccactttcacatttaagttg

The resulting product should be purified, digested with EcoRI and PstI and ligated in pSB vector of choice.

Important: In contrast to the original part, this one has TetR with no LVA-like tag and no transcriptional terminators.
If you want to add terminator, we do recommend part Part:BBa_B0025.

Source

Part:BBa_K145201

References