Part:BBa_K2515002:Experience

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Applications of BBa_K2515002

This part was used by our team to clone all gRNAs needed for our ACE OF BASE project. We also suggest to use the following quick and easy way to test your cloned gRNAs for functional activity. Transform this vector with your gRNA of interest into cells with Cas9 and into cells without Cas9. Having in mind that the combination of gRNA + Cas9 is lethal in E. coli (unless you provide repair matrix too), you should get a lot of colonies on the plate without Cas9 and none on the plate with Cas9 if everything is fine (see the picture). To use this trick your gRNA should tagret a sequence inside the E. coli genome.

User Reviews

UNIQ17d9691c2ad8bd5c-partinfo-00000000-QINU UNIQ17d9691c2ad8bd5c-partinfo-00000001-QINU