Part:BBa_K2510103

lacz-pdDronpa

This part is lacz-mutDronpa fusion, the output is β-galactosidase that can have higher activity levels by shining cyan [fig 2] shows that this copy shows a better control than the original dronpa , the work flow of the β-galactosidase activity experiment is indicated in figure 1

<img id="fig13" src="" />

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                                           Figure 13:   An overview of the experiment done to evaluate the activity of β-galactosidase-Dronpa fusion. 
                                           
                                   

                            <img id="fig14" src="" /> 
                             <img src="">
                                   
                                           Figure 14:  Top: X-Gal grayscale picture, testing the activity of β-galactosidase fusion with both wtDronpa and mutDronpa, indicating that β-galactosidase-mutDronpa fusion is more responsive to cyan light than the β-galactosidase-wtDronp.

Down:90 fold difference in the activity between the MutDronpa caged beta-gal open and closed state after 4 hours of incubation

                                   
                                           
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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]