Coding
Part:BBa_K2510005:Experience
Designed by: Pierre-Luc Satin Group: iGEM17_Paris_Bettencourt (2017-10-02)
The construct was inserted in pSb1C3 and cloned in E. coli BL21
The cell were grown during 4 hours after log phase. We added 0.1% of silicate to the culture. We left the cell overnight.
In the morning we stained them with rhodamine 123 and passed them through flow cytometry.
We can clearly see the same population for the three cell line which have a higher fluorescence than the control. It would mean the presence of polysilicate.
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