Coding

Part:BBa_K2510005:Experience

Designed by: Pierre-Luc Satin   Group: iGEM17_Paris_Bettencourt   (2017-10-02)


The construct was inserted in pSb1C3 and cloned in E. coli BL21 The cell were grown during 4 hours after log phase. We added 0.1% of silicate to the culture. We left the cell overnight. In the morning we stained them with rhodamine 123 and passed them through flow cytometry.

SilicateprodpasteurPB.png

We can clearly see the same population for the three cell line which have a higher fluorescence than the control. It would mean the presence of polysilicate.

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