Coding

Part:BBa_K2510001

Designed by: Oleksandra Sorokina, Aya Goma   Group: iGEM17_Paris_Bettencourt   (2017-10-02)


CARP2

Coral acid-rich protein2(CARP2) is derived from expression of genes obtained from the common stony coral, Stylophora pistillata and was identified to be responsible for calcium carbonate precipitation. CARP2 contain a secretory signal peptide, suggesting that they are discharged from the cells. CARPs 2 contain an isoleucineproline-valine(IPV)-like motif following the signal peptide that has previously been suggested to assist in the trafficking of secreted, acidic, calcium-binding proteins out of the rough endoplasmic reticulum in metazoans.The putative mechanism of calcium carbonate nucleation is that the highly acidic pocket of CARPs localize the substrate, buffer and catalyze the reaction between calcium ion and carboxylate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 985


[edit]
Categories
Parameters
None