Coding

Part:BBa_K2505002:Design

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)


atipt4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 881


Design Notes

This gene is derived from Arabidopsis thaliana and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase ([http://www.genome.jp/dbget-bin/www_bget?ec:2.5.1.112 [EC:2.5.1.112] ]: cytokinin synthase) activity and catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, producing cytokinin nucleotides. Note that cytokinin nucleotides are the immature form.

The DNA sequences of atipt4 optimized for expressing in human cells considering the codon usage.


Source

This gene was synthesized by IDT.

References

Kakimoto, T.(2001). Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases. Plant Cell Physiol, 42:677-85.

Kurakawa, T., Ueda, N., Maekawa, M., Kobayashi, K., Kojima, M., Nagato, Y., ... Kyozuka, J. (2007). Direct control of shoot meristem activity by a cytokinin-activating enzyme. Nature, 445(7128), 652-655.