Coding
Protein E

Part:BBa_K2500006:Design

Designed by: Vasileios Cheras   Group: iGEM17_ETH_Zurich   (2017-10-24)


Protein E: Bacteria-Lysing Protein Encoded by Phage Phi X 17


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid encoding protein E and its sequence were kindly provided by Dr. Irene Wüthrich. The sequence was codon optimized for efficient codon usage by Escherichia coli K-12 strain in Geneious. The parameters of the optimization were adjusted in order to avoid any restriction sites related to BioBrick assembly, namely AgeI, AviII, BamHI, BglII, EcoRI, NgoMIV, NheI, NotI, PstI, PvuII, SapI, SpeI, XbaI, XhoI. The final design was ordered as a gBlock.

Source

Organism: Salmonella typhimurium
Original sequence: Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature Chemical Biology 13.1 (2017): 75-80.
Acquired as: gBlock