Coding
Part:BBa_K2492003:Design
Designed by: ZHOU Xinyu Group: iGEM17_SUSTech_Shenzhen (2017-10-08)
GEM-GECO, a calcium indicator. When bound with calcium, GEMgeco has blue shift of its emission.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1064
Illegal SapI.rc site found at 970
Design Notes
We combine the channel with GEMgeco. The neuron is actived by lighting the channel, the concentration of calcium changes and cause the blue shift of GEMgeco. Thus, we can confirmed that the neuron is actived.
Source
Developed from green calcium indicator.