Project
100-P1-P2

Part:BBa_K2491027:Design

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-10-10)


Combination of Part 1 and Part 2 of Phenanthrene Degradation - 100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4157
    Illegal NheI site found at 4180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 413
    Illegal BsaI.rc site found at 5641


Design Notes

Read P1 and P2 as this sequence is just the ligation of them.

Source

Synthetic Genes based of Burkholderia sp. Strain RP007

References