Coding
101-F1
Part:BBa_K2491002
Designed by: Philippe Hansen-Estruch Group: iGEM17_CCA_San_Diego (2017-10-01)
Part 1 of Fluorene Catabolic Pathway - 101
Composite part consisting of the upper fluorene catabolic pathway synthetic ORFs [flnB (1,1a-dihydroxy-1-hydro-9-fluorenone dehydrogenase), dbfA1 (angular dioxygenase large subunit), and dbfA2 (angular dioxygenase small subunit)] under the control of the constitutive promoter BBa_J23101 and RBS BBa_B0034 and carries the terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 403
Illegal BsaI.rc site found at 1515
[edit]
Categories
Parameters
None |