Coding
100_F1

Part:BBa_K2491000:Design

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-09-30)


Fluorene Degradation Sequence First Part 100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 403
    Illegal BsaI.rc site found at 1515


Design Notes

The catabolic pathway was synthetized as two polycistronic operons with the codon optimized for E.coli expression, eliminating any prefix and suffix restriction sites and any overlapping sequences. In addition, each operon is under the control of its own promoter parts (insert 1 and insert 2) with its own terminator sequence and with RBS sequences inserted between codons.

Source

Synthetic Genes based on Terrabacter sp. DBF63

References