Part:BBa_K2486114

Anti malarial peptide mPLA2 (no stop codon)

[theoretical part] [not assembled, not synthesized, not sent, not characterized]

Anti malarial peptide derived from Apis mellifera phospholipase A2 (1). Modified to not hold enzymatic activities (2) but still be effective. Used in paratransgenic approaches to block Plasmodium transmission in vivo. (3)

GenBank: https://www.ncbi.nlm.nih.gov/nuccore/X16709

1- Moreira, Luciano A., et al. "Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes." Journal of Biological Chemistry 277.43 (2002): 40839-40843.

2 - Rodrigues, F. G., et al. "Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development." Insect molecular biology 17.2 (2008): 175-183.

3 - Wang, Sibao, et al. "Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria." Science 357.6358 (2017): 1399-1402.

Sequence codon optized to E. coli with https://www.idtdna.com/CodonOpt

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]