Coding

Part:BBa_K2486104

Designed by: Tiago Lubiana Alves   Group: iGEM17_USP-Brazil   (2017-10-27)


Anti-Dengue (NS1) P2 nanobody (no stop codon)

[theoretical part] [not assembled, not synthesized, not sent, not characterized]

Nanobody targeting NS1 protein of Dengue serotype 2. This protein helps the virus to invade the mosquito midgut.

GenBank: https://www.ncbi.nlm.nih.gov/nuccore/KF193086.1

1 - : Development of VHH Antibodies against Dengue Virus Type 2 NS1 and Comparison with Monoclonal Antibodies for Use in Immunological Diagnosis Fatima A, Wang H, Kang K, Xia L, Wang Y, et al.

2 - Liu J, Liu Y, Nie K, et al. Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes. Nature microbiology. 2016;1(9):16087. doi:10.1038/nmicrobiol.2016.87.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None