Part:BBa_K2486100

Trypanolytic nanobody Nb_An05 (no stop codon)

[theoretical part] [not assembled, not synthesized, not sent, not characterized]

Nanobody targeting Trypanosoma bursei, the cause of african sleeping sickness, transmitted by the Tse-Tse fly.

GenBank acession: AY263488 https://www.ncbi.nlm.nih.gov/nuccore/AY263488

1 Stijlemans B, Conrath K, Cortez-Retamozo V, Van Xong H, Wyns L, et al. Efficient targeting of conserved cryptic epitopes of infectious agents by single domain antibodies. African trypanosomes as paradigm. J Biol Chem. 2004;279:1256–1261

2 Stijlemans B, Caljon G, Natesan SKA, et al. High Affinity Nanobodies against the Trypanosome brucei VSG Are Potent Trypanolytic Agents that Block Endocytosis. Parsons M, ed. PLoS Pathogens. 2011;7(6):e1002072. doi:10.1371/journal.ppat.1002072.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 50