DNA

Part:BBa_K2486025:Design

Designed by: Cau� Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-28)


16 base pair spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this part to be used in situations where the CDS and terminator are synthesized together, this part would be designed in a smaller version if we used the iGEM 3A assembly, once there would be a scar between the CDS and the terminator.


Source

Escherichia coli K12 MG-1655

References

  1. Rangel-Chavez, C., Galan-Vasquez, E., & Martinez-Antonio, A. (2017). Consensus architecture of promoters and transcription units in Escherichia coli: design principles for synthetic biology. Molecular BioSystems, 13(4), 665-676.