DNA

Part:BBa_K2486024:Design

Designed by: Cau� Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-28)


6 base pair spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this part to be used in situations where the RBS and the CDS are synthesized together, this part would not be necessary if we used the iGEM 3A assembly, once there would be a scar between the RBS and the CDS


Source

Escherichia coli K12 MG-1655

References

  1. Rangel-Chavez, C., Galan-Vasquez, E., & Martinez-Antonio, A. (2017). Consensus architecture of promoters and transcription units in Escherichia coli: design principles for synthetic biology. Molecular BioSystems, 13(4), 665-676.