Device

Part:BBa_K2486021

Designed by: Cau� Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)


PdtxR/DtxR based Fe2+ biosensor

Biosensor constructed by the ligation of parts BBa_K2486021 and BBa_K2486004.

Usage and Biology

We designed this part in order to validate our newly designed promoter PdtxR (BBa_K2486023). The biosensor was able to respond to different concentrations of iron, which suggests it could be used to detect and differentiate Fe2+ content of the blood.

Validation

T--USP-Brazil--Detecttion_Iron_Lactate_dtxR%281%29.png Figure 1 - Promoter activity (GFP/OD600) under different concentrations of Iron Chloride (Fe2+)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 843
    Illegal NheI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1420
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


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Categories
Parameters
None