Composite

Part:BBa_K2486020

Designed by: Cau� Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)


PlldR_tetR/TetR based tetracycline biosensor

A biosensor for tetraccycline

Characterization

T--USP-Brazil--Detecttion_Iron_Lactate_concept_%285%29.PNG

The data of the validation of this part in the presence of LldR and TetR is described in parts BBa_K2486019 (LldR-based lactate biosensor) and BBa_K2486020 (TetR-based tetracycline biosensor) and here.

PlldR_tetR promoter activity in the presence of LldR regulator

800px-T--USP-Brazil--Detecttion_Iron_Lactate_lldR%281%29.png

PlldR_tetR promoter activity in the presence of TetR regulator

T--USP-Brazil--Detecttion_Iron_Lactate_TetR%282%29.png

Conclusion

The presence of LlldR and lactate affects the circuit as expected, repressing it in the absence of lactate. The presence of TetR and tetracycline worked as expected too, sugesting that the part could work with both regulators.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 676
    Illegal NheI site found at 699
    Illegal NheI site found at 835
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1545


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Categories
Parameters
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