Part:BBa_K2486005:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2486005

This part was used in our project as an exogenous iron sensor for E. coli and P. agglomerans. The repressor should detect an increase in Fe2+ levels in the midgut of anophelines fed with blood and regulate the expression of antiparasitic peptides (against Plasmodium spp., as a proof of concept). As dtxR is a repressor in the presence of Fe2+ we have coupled it to a logic inverter (TetR) which ultimately regulates the expression of our system in combination with a lactate detector (LldR). We have selected this regulator instead of the endogenous Fur (ferric uptake regulator) in order to avoid metabolic interferences as there are no DNA binding motifs for dtxR in the genome of our chassis (Boyd, 1998).

User Reviews

UNIQ77bee4c8702e06b2-partinfo-00000000-QINU UNIQ77bee4c8702e06b2-partinfo-00000001-QINU