Coding

Part:BBa_K2486003:Design

Designed by: Felipe Xavier Buson   Group: iGEM17_USP-Brazil   (2017-10-02)


6xHis-CecropinA-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesizing this part already with the His tag and signal peptide war in order to minimize lab flowthrough and possible complications in the cloning/assembly process.


Source

Cecropin A sequence comes from the silk moth Hyalophora cecropia. HlyA sequence comes from type 1 bacterial secretion system.

References