Composite

Part:BBa_K2485100

Designed by: Brian Vuong, Sierra Harken   Group: iGEM17_Stanford-Brown   (2017-07-31)


ompF, 5x Glycine linker, and NanoLuc

This composite part includes an existing NanoLuc construct (BBa_K1159001), with the addition of a 5x Glycine linker. It is conjugated to ompF in this way, such that the two create a fusion protein localized to the outer membrane of E. Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None