Coding

Part:BBa_K2485012

Designed by: Michael Herschl   Group: iGEM17_Stanford-Brown   (2017-09-20)


Proteorhodopsin-cPT-RFP

This is a triple fusion protein containing green light absorbing proteorhodopsin, the cPT region of IcsA, and Fresno RFP. It is intended to function as a unipolarly expressing proton pump. The cPT region directs the localization of the protein to the old pole of E. Coli while the proteorhodopsin pumps protons out of the cell in response to green light (must be grown in 10uM all-trans retinal). RFP has also been tagged for fluorescent visualization. We also have referred to this as the Voltage protein. See BBa_K2485013 for characterization. Note that this part improves upon part BBa_K773002 from the 2012 Caltech iGEM by adding multiple functional domains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



[edit]
Categories
Parameters
None