Translational_Unit

Part:BBa_K2483009:Design

Designed by: Bryan Nowack   Group: iGEM17_Potsdam   (2017-10-19)


IAAM and IAAH yeast optimized under GAL1 control


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2301
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2301
    Illegal NgoMIV site found at 1212
    Illegal AgeI site found at 916
    Illegal AgeI site found at 2086
    Illegal AgeI site found at 2377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3594
    Illegal BsaI.rc site found at 913


Design Notes

Because our other parts (BBa_K2483007 and BBa_K2483008) for this project were also under control of the GAL1 promoter (strong, galactose-induced promoter), we needed to do the same here for it to be a control.

We did not check for illegal restricition sites, so the SpeI sites should have to be mutated out.

Source

The CDS for IAAM and IAAH are from the part BBa_K515100 and codon optimized for yeast. GAL1 and CYC1 are taken from the vector we were working with, which was pYES2/CT.

References