Coding

Part:BBa_K2477009:Design

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


TLP with 6x His-tag 2x GS linker and HLYA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 120
    Illegal AgeI site found at 223
    Illegal AgeI site found at 265
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 407


Design Notes

We had to assemble the tag parts onto the end of the TLP part. A mixture of 3A assembly and removal of the stop codon/scar via PCR was used.


Source

TLP is from Oryza sativa. The tags are from the iGEM DNA kit.

References