Composite

Part:BBa_K2477006:Design

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


Rice TLP With 10x His-tag Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal AgeI site found at 284
    Illegal AgeI site found at 326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 468


Design Notes

The TLP gene had to be ordered as a G-block and assembled into an iGEM backbone using Gibson Assembly.


Source

Oryza sativa is where the TLP gene was taken from.

References