Composite

Part:BBa_K2476001

Designed by: Vivian Hu   Group: iGEM17_Pittsburgh   (2017-10-27)


WT CheY + Dronpa fusion protein regulated by IPTG

Uses an IPTG inducible promoter with RBS (BBa_J04500), WT CheY + Linker/Dronpa/Linker at 60-61 (BBa_K2476012), Dronpa (BBa_K2476010), and a double terminator (BBa_B0015). Will express WT CheY + Dronpa fusion protein when induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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