Device

Part:BBa_K2463001:Design

Designed by: Jack Lawrence   Group: iGEM17_Warwick   (2017-10-22)


LacI Controlled Biofilm and Motility Regulation Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2014
    Illegal BamHI site found at 240
    Illegal BamHI site found at 1398
    Illegal XhoI site found at 1976
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 656
    Illegal NgoMIV site found at 803
    Illegal AgeI site found at 1139
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 313


Design Notes

Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.


Source

Uniprot entries for important proteins:

PLeD Source: [http://www.uniprot.org/uniprot/Q9A5I5 uniprot.org/uniprot/Q9A5I5]

yhjH Source: [http://www.uniprot.org/uniprot/P37646 uniprot.org/uniprot/P37646]

iGEM Registry Parts:

BBa_R0010 - LacI Promoter

BBa_K2463000 - Biofilm and Motility Control Device

Biofilm and Motility Control Device Affiliated Parts:

BBa_B0034 - Ribosome binding site

BBa_K2012002 - PLeD gene (synthesises c-di-GMP)

BBa_C0072 - Mnt repressor

BBa_B1006 - Terminator

BBa_R0073 - Mnt promoter

BBa_K861090 - Yhjh (degrades c-di-GMP)

BBa_B1003 - Second Terminator

References