Part:BBa_K2462000:Design

dehalogenase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 678
  Illegal BglII site found at 1902
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 90
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 233
  

Design Notes

We optimize the sequence of RdhANP in order to make it suitable for the expression of foreign protein in Bacillus megtherium.

Source

Nitratireducror pacificus ,sequence found through tblastn of protein dehalogenase PDB https://www.ncbi.nlm.nih.gov/Structure/pdb/4UR3