Coding

Part:BBa_K2455000:Experience

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-20)


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Applications of BBa_K2455000

YNB E coli growth.png

Growth of E. coli BL21 cells in Yeast Nitrogen Base medium lacking tryptophan. The strains used carried biobricks involved in tryptophan synthesis and/or export under control of an IPTG-inducible T7 promoter. 1.0 mM IPTG was added after 3 hours of growth to induce expression.

YddG: Tryptophan, Tyrosine, Phenylalanine exporter.

AroG: Gene involved in Tryptophan, Tyrosine, and Phenylalanine synthesis.

TrpE: Gene involved in Tryptophan synthesis.

YddG-AroG: Double gene construct carrying the YddG and AroG genes.

YddG-TrpE: Double gene construct carrying the YddG and TrpE genes.

Control: Empty vector

User Reviews

UNIQcff5ac01dd61fd8a-partinfo-00000000-QINU UNIQcff5ac01dd61fd8a-partinfo-00000001-QINU