Part:BBa_K2455000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2455000

Growth of E. coli BL21 cells in Yeast Nitrogen Base medium lacking tryptophan. The strains used carried biobricks involved in tryptophan synthesis and/or export under control of an IPTG-inducible T7 promoter. 1.0 mM IPTG was added after 3 hours of growth to induce expression.

YddG: Tryptophan, Tyrosine, Phenylalanine exporter.

AroG: Gene involved in Tryptophan, Tyrosine, and Phenylalanine synthesis.

TrpE: Gene involved in Tryptophan synthesis.

YddG-AroG: Double gene construct carrying the YddG and AroG genes.

YddG-TrpE: Double gene construct carrying the YddG and TrpE genes.

Control: Empty vector

User Reviews

UNIQba8550d355bf1cd5-partinfo-00000000-QINU UNIQba8550d355bf1cd5-partinfo-00000001-QINU