Generator

Part:BBa_K2451006:Design

Designed by: Matthew Kubis   Group: iGEM17_UT-Knoxville   (2017-10-23)


Rob Generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 431
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
    Illegal NgoMIV site found at 77
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains EcoRI and PstI in the coding sequence. Made with Gibson Assembly. The RBS is immediately flanked by the Rob start codon, which could hamper expression.


Source

Rob was obtained from e. Coli MG1655 genomic DNA. The backbone, RBS, and terminator was amplified from our 2016 part, BBa_K1966003.

References