Generator
Part:BBa_K2451002:Design
Designed by: Matthew Kubis Group: iGEM17_UT-Knoxville (2017-10-23)
MarA Generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 447
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 215
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made with Gibson Assembly. The RBS is immediately flanked by the MarA start codon, which could hamper expression.
Source
MarA was obtained from e. Coli MG1655 genomic DNA. The backbone, RBS, and terminator was amplified from our 2016 part, BBa_K1966003.