Composite
RFC 37

Part:BBa_K245077:Design

Designed by: Špela Miklavič   Group: iGEM09_Slovenia   (2009-10-16)

KSI-DP-LL37-ELST-P4-ELST


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. KSI-DP basic part was added to other parts by cutting front inserts with EcoRI and SpeI and front vectors with EcoRI and XbaI, thus forming scar Xba/Spe NgoMIV (BBa_K245080) beween KSI-DP and another part. This was necessary since BB-NIC-III-HisN vector containing KSI-DP (BBa_K245113) does not contain NgoMIV and BspEI restriction sites in its multiple cloning site (which were usually used to assemble composite parts). There is additional NgoMIV restriction site in the scar since BB-NIC-III-HisN vectors containing other parts (iGEM 2009, team Slovenia) have NgoMIV and BspEI restriction sites in their multiple cloning site (XbaI-NgoMIV-part-BspEI-SpeI).

Source

Composed from KSI-DP-LL37-ELST and P4-ELST intermediate parts according to assembly standard 37.

References