Intermediate
RFC 37

Part:BBa_K245052:Design

Designed by: Špela Miklavič   Group: iGEM09_Slovenia   (2009-10-16)

GCN_P2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. We assembled composite parts by cutting front inserts with EcoI and BspEI and front vectors with EcoI and NgoMIV, thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.

Source

Composed from GCN and P2 basic parts according to assembly standard 37.

References