Composite

Part:BBa_K2448035:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


Psicose Biosensor pPsiA-At PsiR-At with D-Psicose 3-epimerase from Clostridium cellulolyticum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1337
    Illegal XhoI site found at 62
    Illegal XhoI site found at 2618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 791
    Illegal AgeI site found at 2351
    Illegal AgeI site found at 2463
    Illegal AgeI site found at 3252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biosensor was built using the Psicose Biosensor pPsiA-PsiR from Agrobacterium tumefaciens (BBa_K2448025) to which we added downstream the D-Psicose-3-Epimerase from C. cellulolyticum (BBa_K2448021).

Source

DNA synthesis of individual parts and assembly by Golden Gate.

References