Composite

Part:BBa_K2448032:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


Fructose Biosensor pFruB-FruR from Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1262
    Illegal XhoI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 2241
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biosensor was built using the Universal Biosensing Chassis (BBa_K2448023, BBa_K2448024). It is based on the FruR transcription factor from Escherichia coli (BBa_K2448009) and its associated promoter pFruB (BBa_K2448017).

Source

DNA synthesis of individual parts and assembly by Golden Gate.

References