Composite

Part:BBa_K2448028:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


Psicose Biosensor pPsiA-PsiR from Sinorhizobium fredii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1334
    Illegal XhoI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1690
    Illegal AgeI site found at 791
    Illegal AgeI site found at 1124
    Illegal AgeI site found at 2348
    Illegal AgeI site found at 2460
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biosensor was built using the Universal Biosensing Chassis (BBa_K2448023, BBa_K2448024). It is based on the PsiR transcription factor from Sinorhizobium fredii (BBa_K2448007) and its associated promoter pPsiA (BBa_K2448012).

Source

DNA synthesis of individual parts and assembly by Golden Gate.

References