Composite

Part:BBa_K2447016:Design

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-11)


Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2085
    Illegal XhoI site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1614
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutations were introduced to be RFC[10] compatible


Source

TlpA, a transcriptional autorepressor from the virulence plasmid of Salmonella typhimurium.

http://www.nature.com/nchembio/journal/v13/n1/full/nchembio.2233.html?WT.feed_name=subjects_synthetic-biology

References